【R112】ATGScript® 1st Strand cDNA Synthesis Kit (+gDNA wiper)

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1150.0

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1000

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R112

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逆转录酶

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999

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产品简介

ATGScript^® Reverse Transcriptase 是在 M-MLV (H- ) Reverse Transcriptase 基础上经过饱和定点突变得到的全新逆转录酶。经过热点突变，ATGScript^® Reverse Transcriptase 具有更高的热稳定性，使其可用于具有二级结构或 GC 含量较高的 RNA 模板的逆转录，并可获得更多全长的 cDNA。并且 ATGScript^® Reverse Transcriptase 的错配率比 M-MLV (H- ) Reverse Transcriptase 大幅降低，提高了克隆的保真度。通过优势组合突变增强了其与模板的亲和力，特别适合少量模板以及低拷贝基因的逆转录。

ATGScript^® 第一链 cDNA 合成试剂盒基于 ATGScript^® Reverse Transcriptase，包含合成高质量第一链 cDNA 所需的所有成分，适合于后续的 PCR、qPCR 以及 PCR 克隆。产品中的 4 × gDNA wiper Mix 可彻底去除残留的基因组 DNA 污染，保证后续定量结果更加可靠；5 × ATGScript^® RT Mix 包含优化的缓冲体系和 dNTP；Enzyme Mix 包含 ATGScript^® Reverse Transcriptase 和 RNasin。可根据需要，选择 Oligo (dT)₂₃VN、Random hexamers 或基因特异引物作为逆转录引物。

产品组成

组分	R112 ( 100 rxns ) ( 20 μl/rxn )
RNase-free ddH₂O	2 × 1 ml
5 × ATGScript^® RT Mix	400 μl
ATGScript^® Enzyme Mix	200 μl
Oligo (dT)₂₃VN (50 μM)	100 μl
Random hexamers (50 ng/μl)	100 μl
4 × gDNA wiper Mix	300 μl